Morphoanatomical study of the species Zanthoxylum kleinii (R.S. Cowan) P.G. Waterman (Rutaceae).

The order Sapindales is comprised of nine families and in Brazil it is represented by six, including Rutaceae Juss., which constitutes the largest group of this order. A variety of species of Zanthoxylum L. are distributed throughout the country, and among them is the species Zanthoxylum kleinii (R.S. Cowan) P.G. Waterman, which is found in the states of Brazil. This study aimed to characterize the morphoanatomy of the leaf, petiole, rachis, and stem of the species Z. kleinii. Histochemical tests were performed, and the sections were visualized under optical and scanning electron microscopy. The analysis showed that the morphoanatomical characteristics of the species are: hypoestomatic leaflets; stomata classified as anomocytic, tetracytic, and anisocytic; dorsiventral mesophyll; cavities that produce a secretion of lipid nature, present in the leaflet, rachis, and petiole; colleters distributed in the leaf; presence of simple non-glandular trichomes in all structures; and prismatic crystals in the petiole. Histochemical tests indicated the presence of phenolic and lipophilic compounds, mucilage, and lignin. With the result of this research, it was possible to identify the nature of the compounds secreted by the secretory structures of the leaves; in addition, the morphoanatomical characterization of Z. kleinii can provide relevant data for future studies for other organs of the species not yet described. Furthermore, contributing concomitantly with data for the genus, in this way, supporting to differentiate them. RESEARCH HIGHLIGHTS: Ultrastructural features observed by microscopic techniques. Calcium oxalate crystals present in the rachis. Microchemical tests confirmed the presence of colleters in the leaflet.

Potential of Ocotea indecora (Schott) Mez essential oil nanoemulsion in schistosomiasis control: Molluscicidal effects.

Schistosomiasis is a neglected disease transmitted through contaminated water in populations with low basic sanitation. The World Health Organization recommends controlling the intermediate host snails of the Biomphalaria genus with the molluscicide niclosamide. This work aims to evaluate the biocidal potential of the nanoemulsion prepared with the essential oil of Ocotea indecora leaves for the control of the mollusk Biomphalaria glabrata, intermediate host of the Schistosoma mansoni, the etiologic agent of schistosomiasis.

Potential of Ocotea indecora Essential Oil for Controlling Drosophila suzukii: Molecular Predictions for Toxicity and Selectivity to Beneficial Arthropods.

The protection of soft-skinned fruits against Drosophila suzukii has relied primarily on the efficacy of a few synthetic molecules. Despite their short-term efficacy, these molecules can cause environmental pollution, unintendedly affect non-target organisms, and fail to provide sustainable control. The shortfalls of using synthetic pesticides warrant the search for alternatives, such as essential oils extracted from plants, with greater eco-friendlier properties. Here, we chemically characterized and evaluated the toxicity of the essential oil extracted from leaves of Ocotea indecora (Schott) Mez (Lauraceae) against D. suzukii via two exposure pathways (ingestion and contact). We also assessed the selectivity of the essential oil to two predatory natural enemies, Eriopis connexa and Chrysoperla externa and two pollinator bees, Apis mellifera and Partamona helleri. In addition, we conducted in silico predictions to investigate potential interactions between the major compound of the essential oil and the insects' transient receptor potential (TRP) channels. Our chromatographic analysis revealed sesquirosefuran (87%) as the major compound. Higher toxicity to adults of D. suzukii was observed in contact exposure (LC50 = 0.43 µL mL-1) compared to ingestion (LC50 = 0.72 µL mL-1). However, the essential oil did not cause mortality to the non-target organisms tested here, even when applied at 2.20 µL mL-1. Molecular predictions demonstrated that sesquirosefuran binds more stably to the TRP channels of D. suzukii than to those expressed in beneficial arthropods. Collectively, our findings provide the initial framework for the potential use of O. indecora essential oil as a sustainable alternative for managing D. suzukii infestations.

Brazilian Green Propolis' Artepillin C and Its Acetylated Derivative Activate the NGF-Signaling Pathways and Induce Neurite Outgrowth in NGF-Deprived PC12 Cells.

Artepillin C is the most studied compound in Brazilian Green Propolis and, along with its acetylated derivative, displays neurotrophic activity on PC12 cells. Specific inhibitors of the trkA receptor (K252a), PI3K/Akt (LY294002), and MAPK/ERK (U0126) signaling pathways were used to investigate the neurotrophic mechanism. The expression of proteins involved in axonal and synaptic plasticity (GAP-43 and Synapsin I) was assessed by western blotting. Additionally, physicochemical properties, pharmacokinetics, and drug-likeness were evaluated by the SwissADME web tool. Both compounds induced neurite outgrowth by activating the NGF-signaling pathways but through different neuronal proteins. Furthermore, in silico analyses showed interesting physicochemical and pharmacokinetic properties of these compounds. Therefore, these compounds could play an important role in axonal and synaptic plasticity and should be further investigated.

Molluscicidal and Cercaricidal Effects of Myrciaria floribunda Essential Oil Nanoemulsion.

Schistosomiasis is a tropical disease transmitted in an aqueous environment by cercariae from the Schistosoma genus. This disease affects 200 million people living in risk areas around the world. The control of schistosomiasis is realized by chemotherapy, wastewater sanitation, health education, and mollusk control using molluscicidal agents. This work evaluates the effects of a nanoemulsion containing essential oil from Myrciaria floribunda leaves as a molluscicidal and cercaricidal agent against Biomphalaria glabrata mollusks and Schistosoma mansoni cercariae. The Myrciaria floribunda essential oil from leaves showed nerolidol, ß-selinene, 1,8 cineol, and zonarene as major constituents. The formulation study suggested the F3 formulation as the most promising nanoemulsion with polysorbate 20 and sorbitan monooleate 80 (4:1) with 5% (w/w) essential oil as it showed a smaller droplet size of approximately 100 nm with a PDI lower than 0.3 and prominent bluish reflection. Furthermore, this nanoemulsion showed stability after 200 days under refrigeration. The Myrciaria floribunda nanoemulsion showed LC50 values of 48.11 µg/mL, 29.66 µg/mL, and 47.02 µg/mL in Biomphalaria glabrata embryos, juveniles, and adult mollusks, respectively, after 48 h and 83.88 µg/mL for Schistosoma mansoni cercariae after 2 h. In addition, a survival of 80% was observed in Danio rerio, and the in silico toxicity assay showed lower overall human toxicity potential to the major compounds in the essential oil compared to the reference molluscicide niclosamide. These results suggest that the nanoemulsion of Myrciaria floribunda leaves may be a promising alternative for schistosomiasis control.

Poly-ɛ-caprolactone nanocapsules loaded with copaiba essential oil reduce inflammation and pain in mice.

Diverse drugs have been used for the management of inflammation disorders and pain. However, they present many side effects and stimulate the search for new pharmacotherapeutic alternatives. Plant-derived products such as copaiba essential oil (CO) offer beneficial pharmacological effects. On the other hand, essential oil's low water solubility and physical instability hinder its in vivo application. Thus, poly-ɛ-caprolactone (PCL)-based nanocarriers have been used to increase their stability and efficacy. This work aimed to encapsulate CO in PCL nanocapsules and evaluate their effect on inflammation models and pain. The polymeric nanocapsules loading CO (CO-NC) were prepared by nanoprecipitation technique, characterized, and analyzed for their anti-inflammatory effect in vitro and in vivo. The results showed that CO-NC presented a spherical shape, 229.3 ± 1.5 nm diameter, and a negative zeta potential (approximately -23 mV). CO and CO-NC presented anti-inflammatory and antioxidant effects by LPS-activated macrophages (J774 cells). In addition, CO-NC significantly reduced TNF-α secretion (3-fold) compared to CO. In vivo, pre-treatment with CO or CO-NC (50, 100, 200 mg/kg, intraperitoneal; i.p) reduced the mechanical allodynia, paw edema, and pro-inflammatory cytokines induced by intraplantar (i.pl) injection of carrageenan in mice. Specifically, CO-NC (200 mg/kg; i.p.) reduced the production of TNF-α similar to the control group. Our results support using polymeric nanocapsules for CO delivery in inflammatory conditions.

Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from Ocotea indecora (Shott) Mez in Aspergillus Species.

The Aspergillus genus, the etiological agent of aspergillosis, is an important food contaminant and mycotoxin producer. Plant extracts and essential oils are a source of bioactive substances with antimicrobial potential that can be used instead of synthetic food preservatives. Species from the Lauraceae family and the Ocotea genus have been used as traditional medicinal herbs. Their essential oils can be nanoemulsified to enhance their stability and bioavailability and increase their use. Therefore, this study sought to prepare and characterize both nanoemulsion and essential oil from the Ocotea indecora's leaves, a native and endemic species from the Mata Atlântica forest in Brazil, and evaluate the activity against Aspergillus flavus RC 2054, Aspergillus parasiticus NRRL 2999, and Aspergillus westerdjikiae NRRL 3174. The products were added to Sabouraud Dextrose Agar at concentrations of 256, 512, 1024, 2048, and 4096 µg/mL. The strains were inoculated and incubated for up to 96 h with two daily measurements. The results did not show fungicidal activity under these conditions. A fungistatic effect, however, was observed. The nanoemulsion decreased the fungistatic concentration of the essential oil more than ten times, mainly in A. westerdjikiae. There were no significant changes in aflatoxin production.

Molluskicidal nanoemulsion of Neomitranthes obscura (DC.) N. Silveira for schistosomiasis control.

Schistosomiasis is caused by the parasite Schistosoma mansoni, which uses mollusks of the Biomphalaria genus as intermediate hosts. In 2020, approximately 241 million people worldwide underwent treatment for schistosomiasis. For this reason, the World Health Organization encourages research on alternative molluskicides based on plant species. The objective of this work was to investigate Neomitranthes obscura essential oil from leaf chemical composition and its essential oil nanoemulsion activity on intermediate hosts of schistosomiasis Biomphalaria glabrata control. The major chemical components of the Neomitranthes obscura essential oil were zonarene, seline-3,7(11)-diene, ß-selinene, and α-selinene. The nanoemulsion tested using 24-well plate methodology showed lethality and juvenile mollusks with LC90 values of 53.9 and 25.0 ppm after 48 h, respectively, and on their spawning with an LC90 of 66.2 ppm after 48 h. Additionally, the nanoemulsion exhibited an LC90 value against the infective form of the parasite Schistosoma mansoni of 11.5 ppm after 4 h. This pharmaceutical formulation acted inhibiting the acetylcholinesterase activity and was not toxic for Mellanoides sp. This result suggests the use of this nanoformulation as a promising alternative in the control of Biomphalaria glabrata and the transmission of schistosomiasis.

Correlation of the Handgrip Strength and Body Composition Parameters in Young Judokas.

Maximum isometric handgrip strength (MIHS) is a relevant parameter for judo performance and is related to health. Understanding the association between MIHS and MIHS relative (MIHSrel) and the absolute lean mass of the arm (LMarm) and the percentage of body fat (%BF) can provide important information for performance and health. The study aimed to investigate the correlation between MIHS and MIHSrel with the LMarm and the %BF of young judokas (sub-19, 15 males and 14 females). MIHS was measured using a multipurpose digital dynamometer with a load cell for computer. Body composition was measured by dual-energy X-ray absorptiometry (DXA). The correlation between MIHS, MIHSrel with LMarm and %BF was verified through Pearson's correlation, with a significance level of p < 0.05. Correlation between MIHSrel and %BF was very high only in female judokas for both arms (right, r = -0.76; left, r = -0.75). Correlations between LMarm and MIHS of the right and left arms ranged from very high to almost perfect for both sexes (r = 0.74 to 0.94). These results highlight the importance of strengthening the arms in young judokas, and controlling body fat for performance and health, especially in female judokas.

Ethyl acetate fractions of Myrciaria floribunda, Ocotea pulchella, and Ocotea notata exhibit promising in vitro activity against Sporothrix brasiliensis isolates with low susceptibility to itraconazole.

Sporothrix brasiliensis with low susceptibility isolates were described from the Brazilian zoonotic sporotrichosis hyperendemics. The aim of this work was to evaluate distinct fractions of Ocotea pulchella, Ocotea notata, Myrciaria floribunda, and Hypericum brasiliense plant extracts against itraconazole-sensitive and low susceptibility S. brasiliensis isolates. Crude extracts were tested against clinical isolates and the ATCC MYA4823 to determine the minimum inhibitory concentrations (MICs) and fungicidal or fungistatic activities (MFC). A high MICs and MFCs amplitude (1 - > 128 µg/mL) were obtained for seven extracts. The highest antimicrobial activities against sensitive S. brasiliensis were displayed by the ethyl acetate extracts of O. notata (MIC = 2-128 µg/mL) and M. floribunda (MIC = 1-8 µg/mL). A fungicidal effect was observed for all fraction extracts. Ocotea spp. and M. floribunda ethyl acetate extracts provide promising profiles against itraconazole-sensitive or low susceptibility S. brasiliensis. Future studies will determine if these extracts can contribute as alternative therapies to this neglected zoonosis.

Autoetnografia crítica na educação física escolar: do estudo da experiência cultural à reconstrução da prática docente / Critical autoetnography in physical education classes: from the study of cultural experience to the reconstruction of teaching practice / Autoetnografía crítica en la educación física escolar: del estudio de la experiencia cultural a la reconstrucción de la práctica docente

A discussão deste texto, elaborada a partir de uma pesquisa de doutorado realizada em uma escola pública municipal do Rio Grande do Sul, tem por objetivo compreender como a autoetnografia crítica constitui um processo teórico-metodológico de pesquisa que permite reconstruir a prática docente. Para isso, apresentamos o aporte teórico-metodológico da autoetnografia crítica ao relacionar a pesquisa autoetnográfica com a fundamentação conceitual da teoria crítica. Na sequência, utilizamos narrativas de experiências docentes para identificar como os processos recursivos e reflexivos, ambos sustentados na reflexão crítica, têm implicações diretas na formação e na prática docente do pesquisador. Nas considerações finais, dialogamos sobre a autoetnografia crítica como experiência duplamente transformadora, pois envolve processos gradativos e simultâneos de leituras de mundo e reconstrução da prática docente que ocorrem ao longo da própria investigação. (AU)

The discussion of this text, elaborated from a doctoral research project carried out in a municipal public school in Rio Grande do Sul, aims to understand how critical autoethnography constitutes a theoretical-methodological research process that allows the reconstruction of the teaching practice. For this, we present the theoretical-methodological contribution of critical autoethnography by relating autoethnographic research with the conceptual foundation of critical theory. , We then use narratives of teaching experiences to identify how recursive and reflective processes, both supported by critical reflection, have direct implications for the researcher's teaching training and practice. In the final considerations, we discuss critical autoethnography as a doubly transforming experience, as it involves gradual and simultaneous processes of reading the world and reconstructing the teaching practice that occurred throughout the investigation itself. (AU)

La discusión de este texto, elaborado a partir de una investigación doctoral realizada en una escuela pública municipal de Rio Grande do Sul, pretende comprender cómo la autoetnografía crítica constituye un proceso de investigación teórico-metodológico que permite la reconstrucción de la práctica docente. Para ello, presentamos el aporte teórico-metodológico de la autoetnografía crítica al relacionar la investigación autoetnográfica con la fundamentación conceptual de la teoría crítica. En la secuencia, utilizamos narrativas de experiencias docentes para identificar cómo los procesos recursivos y reflexivos, ambos sustentados en la reflexión crítica, tienen implicaciones directas en la formación y práctica docente del investigador. En las consideraciones finales, nosotros discutimos la autoetnografía crítica como una experiencia doblemente transformadora, en tanto implica procesos graduales y simultáneos de lectura del mundo y de reconstrucción de la práctica docente que se dan a lo largo de la propia investigación. (AU)

Myrciaria Genus: Bioactive Compounds and Biological Activities.

The Myrtaceae family is of angiosperms, imposing its size and economic, cultural, and scientific importance. The genus Myrciaria, belonging to this family, has 33 species currently accepted, many of which are research targets aimed at elucidating their bioactive compounds and biological activities. Most species of the Myrciaria genus have terpenes in their composition, mainly mono and sesquiterpenes, and phenolic compounds such as tannins, phenolic acids, and flavonoids. Other secondary metabolites are also observed, such as alkaloids, steroids, coumarins, saponins, and naphthoquinones. These bioactive compounds are closely related to these species' most diverse biological activities: antioxidant, anti-inflammatory, analgesic, antiproliferative, antimicrobial, antiparasitic, insecticide, metabolic, protective, and nutraceutical. This work aims to provide a review of secondary metabolites and medicinal properties related to the genus Myrciaria, thus stimulating further studies on the species of this genus.

In vitro comparison between antimicrobial and antibiofilm effects of Green Propolis and Baccharis dracunculifolia against Staphylococcus pseudintermedius isolate.

Staphylococcus pseudintermedius is the leading cause of canine pyoderma. Honeybee products are common to treat this and other types of infections. High average annual population loss of bees has been observed. This study evaluated antibacterial and antibiofilm profile of Green Propolis and Baccharis dracunculifolia against S. pseudintermedius and the chemical similarities among both. Ethanolic extracts were produced and chemically characterized. The isolates were subjected to treatment with the extracts in both planktonic and sessile forms. Green propolis minimum inhibitory concentration (MIC) was 0.156 mg / mL, and minimum bactericidal concentration (MBC) was 0.312mg / mL. Baccharis dracunculifolia extract MIC and MBC was 0.312mg / mL and 2.5 mg / mL, respectivelly. Both extracts reduced SD55 formation of biofilm at minimum inhibitory concentration and at 1/8 minimum inhibitory concentration. The results observed in relation to ED99, were similar for both extracts. Besides that, similar chemical indicators between both extracts, including the presence of Artepellin C, suggest that the Baccharis dracunculifolia extract could be an alternative to the Green Propolis extract in the treatment of staph infections.

Nanoemulsion of Sideroxylon obtusifolium as an Alternative to Combat Schistosomiasis.

Schistosomiasis is caused by the intestinal parasite Schistosoma mansoni. Individuals are affected by schistosomiasis when they are exposed to aquatic environments contaminated with Schistosoma cercariae that emerged from the infected intermediate host mollusk of the genus Biomphalaria. The WHO recommends using molluscicidal products to reduce the snail population and disease transmission. The WHO encourages the search for alternative substances in schistosomiasis control. Natural products are seen as a promising alternative because they are abundant in countries where schistosomiasis is endemic and have many different substances in their extracts, impairing cases of resistance. Therefore, the nanoemulsion effect of a butanol-soluble fraction of Sideroxylon obtusifolium leaves was evaluated against three study points in the biological cycle of the disease, that is, adults and young Biomphalaria glabrata, spawning by the host mollusk, and infectious larvae of the parasite. Extract-SOB (butanol fraction) and nano-SOB (nanoemulsion) demonstrated promising activity in adult B. glabrata population control with an LC50 of 125.4 mg/L, an LC90 of 178.1 mg/L, an LC50 of 75.2 mg/L, and an LC90 of 97 mg/L. Nano-SOB presented greater potency against young B. glabrata, with an LC90 of 72.1 mg/L and an LC50 of 58.3 mg/L. Still, relevant activity against S. mansoni cercariae was eliminated in 4 h (LC90: 34.6 mg/L). Nano-SOB reduced viable spawning by approximately 30% at 178.1 and 97 mg/L. Referring to most substances in this extract, quercetin-3-rhamnosyl-(1-6)-galactoside and hyperoside may cause low environmental toxicity and human toxicity according to in silico analysis. Thus, nano-SOB is a promising agent to combat B. glabrata population growth and schistosomiasis transmission.

Eugenia sulcata (Myrtaceae) Nanoemulsion Enhances the Inhibitory Activity of the Essential Oil on P2X7R and Inflammatory Response In Vivo.

P2X7R is a purinergic receptor with broad expression throughout the body, especially in immune system cells. P2X7R activation causes inflammatory mediators to release, including interleukin-1ß (IL-1ß), the processing and release of which are critically dependent on this ion channel activation. P2X7R's therapeutic potential augments the discovery of new antagonistic compounds. Thus, we investigated whether the Eugenia sulcata essential oil could block P2X7R activity. The essential oil (ESO) dose-dependently inhibited ATP-promoted PI uptake and IL-1ß release with an IC50 of 113.3 ± 3.7 ng/mL and 274 ± 91 ng/mL, respectively, and the essential oil nanoemulsion (ESON) improved the ESO inhibitory effect with an IC50 of 81.4 ± 7.2 ng/mL and 62 ± 2 ng/mL, respectively. ESO and ESON reversed the carrageenan-activated peritonitis in mice, and ESON exhibited an efficacy higher than ESO. The majority substance from essential oil, ß-caryophyllene, impaired the ATP-evoked PI uptake and IL-1ß release with an IC50 value of 26 ± 0.007 ng/mL and 97 ± 0.012 ng/mL, respectively. Additionally, ß-caryophyllene reduced carrageenan-induced peritonitis, and the molecular modeling and computational simulation predicted the intermolecular interactions in the P2X7R situs. In silico, results indicated ß-caryophyllene as a potent allosteric P2X7R antagonist, although this substance may present toxic effects for humans. These data confirm the nanoemulsion of essential oil from E. sulcata as a promisor biotechnology strategy for impaired P2X7R functions and the inflammatory response.

Reconectar a educação física à escola: um desafio pós-pandêmico / Reconnecting physical education to school: a post pandemic challenge / Reconectar la educación física a la escuela: un reto post pandemia

Neste texto partimos da reflexão sobre os discursos higienistas atualmente utilizados no contexto social para legitimar a presença da Educação Física na escola em função de um cenário pandêmico. Por conseguinte, evidenciamos que tal discurso é incoerente com o que se espera da formação escolar, principalmente se observarmos os ataques sofridos pela Educação Física na escola e o fato de que esses discursos demonstram o retrocesso da área, marcado pela noção de Educação Física apenas como momento de fazer atividade física. Defendemos uma Educação Física escolar crítica, como possibilidade viável de reconectar a Educação Física, como área de conhecimento, à escola ­ objetivo deste ensaio.

The discussion in this article starts from the reflection on the hygienist discourses which are currently used in the social context and it mains to legitimize the presence of Physical Education at school in a pandemic scenario. We evidence that such discourse is inconsistent in relation to what is expected by school education, especially if we observe the the attacks suffered by Physical Education at school and the fact that these discourses demonstrate a step backwards, marked by the notion which consider Physical Education as time to practice a physical activity. The aim of this essay is defending critical school Physical Education as a viable possibility to reconnect this area of knowledge to the school.

Este estudio forma parte de la reflexión de los discursos higienistas actualmente utilizados en el contexto social para legitimar la presencia de la Educación Física en la escuela ante un escenario de pandemia. Por lo tanto, evidenciamos que tal discurso es incoherente con lo que esperamos de la formación escolar sobre todo si se observan los ataques sufridos por la Educación Físicaen la escuela y demostramos que estos discursos la regresión del área, marcada por la noción de Educación Física como tiempo para hacer actividad física. Defendemos la Educación Física escolar crítica como una posibilidad viable para reconectar la Educación Física, como área de conocimiento, a la escuela ­ objetivo de este ensayo.

Reconectar a educação física à escola: um desafio pós-pandêmico / Reconnecting physical education to school: a post pandemic challenge / Reconectar la educación física a la escuela: un reto post pandemia

Neste texto partimos da reflexão sobre os discursos higienistas atualmente utilizados no contexto social para legitimar a presença da Educação Física na escola em função de um cenário pandêmico. Por conseguinte, evidenciamos que tal discurso é incoerente com o que se espera da formação escolar, principalmente se observarmos os ataques sofridos pela Educação Física na escola e o fato de que esses discursos demostram o retrocesso da área, marcado pela noção de Educação Física apenas como momento de fazer atividade física. Defendemos uma Educação Física escolar crítica, como possibilidade viável de reconectar a Educação Física, como área de conhecimento, à escola ­ objetivo deste ensaio-

The discussion in this article starts from the reflection on the hygienist discourses which are currently used in the social context and it mains to legitimize the presence of Physical Education at school in a pandemic scenario. We evidence that such discourse is inconsistent in relation to what is expected by school education, especially if we observe the the attacks suffered by Physical Education at school and the fact that these discourses demonstrate a step backwards, marked by the notion which consider Physical Education as time to practice a physical activity. The aim of this essay is defending critical school Physical Education as a viable possibility to reconnect this area of knowledge to the school (AU).

Este estudio forma parte de la reflexión de los discursos higienistas actualmente utilizados en el contexto social para legitimar la presencia de la Educación Física en la escuela ante un escenario de pandemia. Por lo tanto, evidenciamos que tal discurso es incoherente con lo que esperamos de la formación escolar sobre todo si se observan los ataques sufridos por la Educación Físicaen la escuela y demostramos que estos discursos la regresión del área, marcada por la noción de Educación Física como tiempo para hacer actividad física. Defendemos la Educación Física escolar crítica como una posibilidad viable para reconectar la Educación Física, como área de conocimiento, a la escuela ­ objetivo de este ensayo (AU).

Essential oils from Ocotea species: Chemical variety, biological activities and geographic availability.

This review describes the chemical composition and biological properties of essential oils from plants of the Ocotea genus, from different origin. This taxon belongs to the Laureaceae family, which in turn, is best known for medicinal use, often related to the essential oils. The text includes studies about Ocotea species distributed mainly on the South American continent, but also reporting species located in North America and Africa. Brazil, Colombia, Costa Rica and Ecuador are countries with highest number of species mentioned in the review. Also, the major components of each essential oil, its chemical structures, as well as the description and extent of biological activities related to essential oils are detailed in this review. Finally, the text discusses the chemical and biological aspects of these studies in a comparatively way, also informing additional data such as yield, biome of origin and pharmacobotanical location.

'Hypericum brasiliense: bactericidal and antibiofilm activity against Staphylococcus aureus in vitro'.

Staphylococcus aureus is considered the most common opportunistic pathogen in humans, capable of forming biofilm, increasing the chances of antibiotic resistance and causes several chronic diseases. Biodiversity is a source of inspiration in the search for new agents against these microorganisms. Hitherto, the efficacy of Hypericum sp. extracts as an antibacterial agent has already been demonstrated against Gram-positive and Gram-negative bacteria. In this study, we observed that until 4 µg/mL, the Hypericum brasiliense extract showed bactericidal activity against a clinical multidrug-resistant S. aureus strain (HU25) and also inhibited biofilm formation at 1/2xMIC (confirmed by SEM) and 1/4xMIC. The extract was also proportionally active against 6 h-preformed biofilm to its concentration (1/2xMIC, 1/4xMIC, p value ≤ 0.05). These promising results make Hypericum brasiliense extract a strong candidate to treat S. aureus infections, including anti-biofilm therapy.

Seroprevalence and risk factors associated with seropositivity for equine encephalomyelitis virus in horses in Rio Grande do Norte, Brazil

The present objective was to investigate the presence of anti-equine viral encephalomyelitis (EVE) antibodies and the possible risk factors for its dissemination in horses raised in the East and West Potiguar mesoregions of the state of Rio Grande do Norte, Brazil. Serological diagnosis for neutralizing antibodies against Eastern (EEEV), Western (WEEV) and Venezuelan (VEEV). Equine viral encephalomyelitis was performed using a seroneutralization technique on 811 blood samples from horses from ninety properties and sixteen municipalities between July 2018 and February 2019. Factors associated with EVE were evaluated using an investigative epidemiological questionnaire, and the data were statistically analyzed using the Epi Info 3.5.2 software with a confidence level of 95%. The seroprevalence of anti-EVE antibodies was 14.2% (115), with 10.36% (84) for EEEV, 6.9% (56) for WEEV, and null for EVE. When analyzing risk factors, it can be concluded that horses raised in properties that do not clean installations and/or rent out their pasture are more likely to have anti-EVE antibodies. These results show evidence that horses raised in the East and West Potiguar mesoregions were exposed to EEEV and WEEV, thus reinforcing the importance of vaccination and serological survey of nonvaccinated horses as a means of monitoring the disease.